Product Info Summary
SKU: | M01072-1 |
---|---|
Size: | 100 μl |
Reactive Species: | Human, Mouse, Rat |
Host: | Rabbit |
Application: | Flow Cytometry, IF, IHC, ICC, WB |
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Product info
Product Name
Anti-alpha smooth muscle Actin ACTA2 Rabbit Monoclonal Antibody
View all alpha-Smooth Muscle Actin Antibodies
SKU/Catalog Number
M01072-1
Size
100 μl
Form
Liquid
Description
Boster Bio Anti-alpha smooth muscle Actin ACTA2 Rabbit Monoclonal Antibody catalog # M01072-1. Tested in WB, IHC, ICC/IF, Flow Cytometry applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-alpha smooth muscle Actin ACTA2 Rabbit Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M01072-1)
Host
Rabbit
Contents
Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.
Clonality
Monoclonal
Clone Number
EO-1
Isotype
Rabbit IgG
Immunogen
A synthesized peptide derived from human alpha smooth muscle Actin
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
M01072-1 is reactive to ACTA2 in Human, Mouse, Rat
Applications
M01072-1 is guaranteed for Flow Cytometry, IF, IHC, ICC, WB Boster Guarantee
Observed Molecular Weight
42 kDa
Calculated molecular weight
42.009kDa
Background of alpha-Smooth Muscle Actin
Activated by icilin, eucalyptol, menthol, cold and modulation of intracellular pH. Involved in menthol sensation. Permeable for monovalent cations sodium, potassium, and cesium and divalent cation calcium.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Innovating Scientists Reward
If you are the first to review this product, or if you have results for a special sample, species or application this product is not validated in, share your results with us and receive product credits you can use towards any Boster products! Applicable to all scientists worldwide.
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Assay dilution & Images
Reconsitution
Restore with deionized water (or equivalent) for reconstitution volume of 1.0 mL
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:1000-1:10000
IHC 1:500-1:1000
ICC/IF 1:100-1:500
FC 1:20
Validation Images & Assay Conditions
Click image to see more details
Figure 1. Western blot analysis of ACTA2 using anti-ACTA2 antibody (M01072-1).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human A549 whole cell lysates,
Lane 2: human Hela whole cell lysates,
Lane 3: human 293T whole cell lysates,
Lane 4: human HepG2 whole cell lysates,
Lane 5: human Caco-2 whole cell lysates,
Lane 6: human SH-SY5Y whole cell lysates,
Lane 7: human U251 whole cell lysates,
Lane 8: human MCF-7 whole cell lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ACTA2 antigen affinity purified monoclonal antibody (Catalog # M01072-1) at 1:1000 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for ACTA2 at approximately 42 kDa. The expected band size for ACTA2 is at 42 kDa.
Click image to see more details
Figure 2. Western blot analysis of ACTA2 using anti-ACTA2 antibody (M01072-1).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: rat heart tissue lysates,
Lane 2: rat lung tissue lysates,
Lane 3: rat brain tissue lysates,
Lane 4: mouse heart tissue lysates,
Lane 5: mouse lung tissue lysates,
Lane 6: mouse brain tissue lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ACTA2 antigen affinity purified monoclonal antibody (Catalog # M01072-1) at 1:1000 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for ACTA2 at approximately 42 kDa. The expected band size for ACTA2 is at 42 kDa.
Click image to see more details
Immunohistochemical analysis of paraffin-embedded human colon, using alpha smooth muscle Actin Antibody.
Click image to see more details
Immunofluorescent analysis of A431 cells, using alpha smooth muscle Actin Antibody .
Protein Target Info & Infographic
Gene/Protein Information For ACTA2 (Source: Uniprot.org, NCBI)
Gene Name
ACTA2
Full Name
Actin, aortic smooth muscle
Weight
42.009kDa
Superfamily
actin family
Alternative Names
1a4 sma; 1a4 smooth muscle actin; AAT6; ACTA2; Actin alpha 2; actin, alpha 2, smooth muscle, aorta; actin, aortic smooth muscle; ACTSA; ACTVS; alpha 2 actin; alpha sma 1a4; alpha SMA; alpha smooth muscle actin; alpha-actin-2; alpha-SMA; alphaSmooth Muscle Actin; alpha-Smooth Muscle Actin; a-sma; Cell growth-inhibiting gene 46 protein; growth-inhibiting gene 46; MYMY5; sma 1a4; SMA; smooth muscle actin ACTA2 ACTSA actin alpha 2, smooth muscle actin, aortic smooth muscle|actin, alpha 2, smooth muscle, aorta|alpha-cardiac actin|cell growth-inhibiting gene 46 protein
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on ACTA2, check out the ACTA2 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for ACTA2: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-alpha smooth muscle Actin ACTA2 Rabbit Monoclonal Antibody (M01072-1)
Hello CJ!
M01072-1 has been cited in 72 publications:
*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.
Estrogen receptor beta maintains expression of KLF15 to prevent cardiac myocyte hypertrophy in female rodents
Fang L,Wang KK,Zhang PF,Li T,Xiao ZL,Yang M,Yu ZX.Nucleolin promotes Ang II-induced phenotypic transformation of vascular smooth muscle cells by regulating EGF and PDGF-BB.J Cell Mol Med.2020 Jan;24(2) :1917-1933.doi:10.1111/jcmm.14888.Epub 2020 Jan 1.PMI
Species: Rat
M01072-1 usage in article: APP:WB, SAMPLE:VSMCS, DILUTION:NA
Shen J,Wei W,Wang X,Yang J,Lu L,Lv X,Xue X.Proliferation of Vascular Smooth Muscle Cells under ox-LDL Is Regulated by Alismatis rhizoma Decoction via InhibitingERK1/2 and miR-17∼92a Cluster Activation.Evid Based Complement Alternat Med.2020 Aug 22;2020:72
Species: Rat
M01072-1 usage in article: APP:IHC, SAMPLE:VSMCS, DILUTION:1:1000
Xu T,Pan L,Li L,Hu S,Zhou H,Yang C,Yang J,Li H,Liu Y,Meng X,Li J.MicroRNA-708 modulates Hepatic Stellate Cells activation and enhances extracellular matrix accumulation via direct targeting TMEM88. J Cell Mol Med. 2020 Jul;24(13):7127-7140.doi: 10.1111/jc
Species: Human
M01072-1 usage in article: APP:WB, SAMPLE:LX-2 CELLS, DILUTION:1:1000
Wang R, Wu G, Dai T, Lang Y, Chi Z, Yang S, Dong D. Naringin attenuates renal interstitial fibrosis by regulating the TGF-β/Smad signaling pathway and inflammation. Exp Ther Med. 2021 Jan;21(1):66. doi: 10.3892/etm.2020.9498. Epub 2020 Nov 23. PMID: 33365
Species: Rat
M01072-1 usage in article: APP:IF, SAMPLE:KIDNEY TISSUE, DILUTION:1:70
Liao J, Wu Q, Qian C, Zhao N, Zhao Z, Lu K, Zhang S, Dong Q, Chen L, Li Q, Du Y. TRPV4 blockade suppresses atrial fibrillation in sterile pericarditis rats. JCI Insight. 2020 Oct 29:137528. doi: 10.1172/jci.insight.137528. Epub ahead of print. PMID: 33119
Species: Rat
M01072-1 usage in article: APP:IHC, SAMPLA:ATRIA TISSUE, DILUTION:1:50
Yang J, Li J, Tan R, He X, Lin X, Zhong X, Fan J, Wang L. Protocatechualdehyde attenuates obstructive nephropathy through inhibiting lncRNA9884 induced inflammation. Phytother Res. 2020 Oct 28. doi:10.1002/ptr.6 919. Epub ahead of print. PMID: 33118280.
Species: Mouse
M01072-1 usage in article: APP:WB, SAMPLE:MOUSE KIDNEY TISSUES, DILUTION:1:1000
Small-diameter hybrid vascular grafts composed of polycaprolactone and polydioxanone fibers
Extracorporeal shock wave therapy with low-energy flux density inhibits hypertrophic scar formation in an animal model
The Role and Mechanism of ?-Klotho in the Calcification of Rat Aortic Vascular Smooth Muscle Cells
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